New Interface for Purification of Proteins: One-Dimensional TiO2 Nanotubes Decorated by Fe3O4 Nanoparticles
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F17%3A39911337" target="_blank" >RIV/00216275:25310/17:39911337 - isvavai.cz</a>
Result on the web
<a href="http://pubs.acs.org/doi/abs/10.1021/acsami.7b08445" target="_blank" >http://pubs.acs.org/doi/abs/10.1021/acsami.7b08445</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acsami.7b08445" target="_blank" >10.1021/acsami.7b08445</a>
Alternative languages
Result language
angličtina
Original language name
New Interface for Purification of Proteins: One-Dimensional TiO2 Nanotubes Decorated by Fe3O4 Nanoparticles
Original language description
In this work, a high surface area interface, based on anodic one-dimensional (1D) TiO2 nanotubes homogeneously decorated by Fe3O4 nanoparticles (TiO2NTs@Fe3O4NPs is reported for the first time for an unprecedented purification of His-tagged recombinant proteins. Excellent purification results were achieved from the model protein mixture, as well as from the whole cell lysate (with His -tagged ubiquitin). Compared to a conventional immobilized -metal affinity chromatography (IMAC) system, specific isolation of selected His -tagged proteins on behalf of other proteins was significantly enhanced on TiO2NTs@Fe3O4NPs interface under optimized binding and elution conditions. The combination of specific isolation properties, magnetic features, biocompatibility, and ease of preparation of this material consisting of two basic metal oxides makes it a suitable candidate for future purification of recombinant proteins in biotechnology. The principally new: material bears a large potential to open new pathways for discoveries in nanobiotechnology and nanomedicine.
Czech name
—
Czech description
—
Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ACS Applied Materials & Interfaces
ISSN
1944-8244
e-ISSN
—
Volume of the periodical
9
Issue of the periodical within the volume
34
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
"28233–28242"
UT code for WoS article
000409395500012
EID of the result in the Scopus database
—