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UHPSFC/ESI-MS Analysis of Lipids

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F18%3A39913647" target="_blank" >RIV/00216275:25310/18:39913647 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1007/978-1-4939-7592-1_5" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-7592-1_5</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/978-1-4939-7592-1_5" target="_blank" >10.1007/978-1-4939-7592-1_5</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    UHPSFC/ESI-MS Analysis of Lipids

  • Original language description

    This new analytical approach for high-throughput and comprehensive lipidomic analysis of biological samples using ultrahigh-performance supercritical fluid chromatography (UHPSFC) with electrospray ionization–mass spectrometry (ESI-MS) is based on lipid class separation using 1.7 μm particle bridged ethylene hybrid silica columns and a gradient of methanol–water–ammonium acetate mixture as a modifier. The method enables a fast separation of 30 nonpolar and polar lipid classes within 6-min analysis time covering six main lipid categories including fatty acyls, glycerolipids, glycerophospholipids, sphingolipids, sterols, and prenols. Individual lipid species within lipid classes are identified based on positive- and negative-ion full scan and tandem mass spectra measured with high mass accuracy and high resolving power. The method is used for the quantitative analysis of lipid species in biological tissues using internal standards for each lipid class. This high-throughput, comprehensive, and accurate UHPSFC/ESI-MS method is suitable for the lipidomic analysis of large sample sets in clinical research.

  • Czech name

  • Czech description

Classification

  • Type

    C - Chapter in a specialist book

  • CEP classification

  • OECD FORD branch

    10406 - Analytical chemistry

Result continuities

  • Project

    <a href="/en/project/LL1302" target="_blank" >LL1302: Mass Spectrometry in Cancer Research: Lipid Biomarkers for Early Diagnostics</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Book/collection name

    Clinical Metabolomics : Methods and Protocols

  • ISBN

    978-1-4939-7592-1

  • Number of pages of the result

    10

  • Pages from-to

    73-82

  • Number of pages of the book

    389

  • Publisher name

    Humana Press

  • Place of publication

    Totowa

  • UT code for WoS chapter