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Determination of acid dissociation constants, enthalpy, entropy andGibbs free energy of the baricitinib by the UV-metric and pH-metricanalysis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F20%3A39916312" target="_blank" >RIV/00216275:25310/20:39916312 - isvavai.cz</a>

  • Alternative codes found

    RIV/00216275:25530/20:39916312

  • Result on the web

    <a href="https://www.sciencedirect.com/science/article/pii/S0731708520314187?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0731708520314187?via%3Dihub</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.jpba.2020.113532" target="_blank" >10.1016/j.jpba.2020.113532</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Determination of acid dissociation constants, enthalpy, entropy andGibbs free energy of the baricitinib by the UV-metric and pH-metricanalysis

  • Original language description

    Baricitinib is a drug used for the treatment of rheumatoid arthritis. It is a selective and reversible inhibitorof Janus kinases 1 and 2, which play an important role in signalling the pro-inflammatory pathwayactivated in autoimmune disorders such as rheumatoid arthritis. The pH-spectrophotometric and pH-potentiometric titrations allowed the measurement of three or four successive dissociation constantsof Baricitinib. Baricitinib neutral LH2molecule was able to protonate into two soluble cations LH42+,LH3+and dissociate into two soluble anions LH−and L2-in pure water. The graph of molar absorptioncoefficients of differently protonated species versus wavelength indicated that the spectra L, LH, LH2were the nearly the same for these species and that the spectra LH4and LH3were also similar. In thepH range from 2–13, four pKa´s of spectra analysis were reliably estimated by REACTLAB at I =0.0020 mol.dm-3values pKTa1= 3.07, pKTa2= 3.87, pKTa3= 6.27, pKTa4= 12.78 at 25◦C and pKTa1= 3.00, pKTa2= 3.79,pKTa3= 6.12, pKTa4= 12.75 at 37◦C. Potentiometric pH-titration analysis for a higher concentration of 1× 10-3mol. dm-3estimated with ESAB at I =0.0001 mol. dm-3values pKTa1= 3.69, pKTa2= 3.81, pKTa3=4.73 at 25◦C and pKTa1= 3.62, pKTa2= 3.73, pKTa3= 4.43 at 37◦C. Molar enthalpy H◦, molar entropy S◦and Gibbs free energy G◦were calculated from the spectra using a dependence ln K to 1/T.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10406 - Analytical chemistry

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2020

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Journal of Pharmaceutical and Biomedical Analysis

  • ISSN

    0731-7085

  • e-ISSN

  • Volume of the periodical

    191

  • Issue of the periodical within the volume

    November

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    11

  • Pages from-to

    113532

  • UT code for WoS article

    000579528000006

  • EID of the result in the Scopus database