Ultrahigh-performance supercritical fluid chromatography / mass spectrometry in the lipidomic analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F22%3A39918849" target="_blank" >RIV/00216275:25310/22:39918849 - isvavai.cz</a>
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S0165993622000292" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0165993622000292</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.trac.2022.116546" target="_blank" >10.1016/j.trac.2022.116546</a>
Alternative languages
Result language
angličtina
Original language name
Ultrahigh-performance supercritical fluid chromatography / mass spectrometry in the lipidomic analysis
Original language description
Ultrahigh-performance supercritical fluid chromatography/mass spectrometry (UHPSFC/MS) is one of the fastest analytical approaches for high-throughput lipidomic analysis covering a wide range of lipid categories and classes. Lipid class separation and lipid species separation are two main separation modes available for the UHPSFC analysis of lipids. The mechanism of the lipid class separation mode is com-parable to that of hydrophilic interaction liquid chromatography (HILIC). The direct comparison of HILIC -ultrahigh-performance liquid chromatography (HILIC-UHPLC) and UHPSFC shows the applicability of UHPSFC/MS for high-throughput quantitation of both nonpolar and polar lipid classes, the faster analysis time, and comparable reproducibility and sensitivity. The retention mechanism of the lipid species separation mode is comparable to reversed-phase UHPLC, but the nonpolar character of supercritical carbon dioxide (scCO(2)) provides a different selectivity compared to that of conventional RP-UHPLC. The introduction of various hyphenation interfaces to MS broadens the applicability and sensitivity range of UHPSFC/MS using scCO(2) as the main component of the mobile phase. The addition of ionic additives (volatile salts, acids, and bases) to the modifier improves the separation of medium to polar lipids. Furthermore, UHPSFC/MS has also a great potential in the chiral separation of lipids, but the field has not yet been fully explored.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA21-20238S" target="_blank" >GA21-20238S: Multidimensional chromatography - mass spectrometry quantitative workflows in detailed characterization of human plasma lipidome</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
TrAC - Trends in Analytical Chemistry
ISSN
0165-9936
e-ISSN
1879-3142
Volume of the periodical
149
Issue of the periodical within the volume
April
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
10
Pages from-to
116546
UT code for WoS article
000790927700001
EID of the result in the Scopus database
2-s2.0-85124286290