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Ultrahigh-performance supercritical fluid chromatography / mass spectrometry in the lipidomic analysis

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F22%3A39918849" target="_blank" >RIV/00216275:25310/22:39918849 - isvavai.cz</a>

  • Result on the web

    <a href="https://www.sciencedirect.com/science/article/pii/S0165993622000292" target="_blank" >https://www.sciencedirect.com/science/article/pii/S0165993622000292</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1016/j.trac.2022.116546" target="_blank" >10.1016/j.trac.2022.116546</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Ultrahigh-performance supercritical fluid chromatography / mass spectrometry in the lipidomic analysis

  • Original language description

    Ultrahigh-performance supercritical fluid chromatography/mass spectrometry (UHPSFC/MS) is one of the fastest analytical approaches for high-throughput lipidomic analysis covering a wide range of lipid categories and classes. Lipid class separation and lipid species separation are two main separation modes available for the UHPSFC analysis of lipids. The mechanism of the lipid class separation mode is com-parable to that of hydrophilic interaction liquid chromatography (HILIC). The direct comparison of HILIC -ultrahigh-performance liquid chromatography (HILIC-UHPLC) and UHPSFC shows the applicability of UHPSFC/MS for high-throughput quantitation of both nonpolar and polar lipid classes, the faster analysis time, and comparable reproducibility and sensitivity. The retention mechanism of the lipid species separation mode is comparable to reversed-phase UHPLC, but the nonpolar character of supercritical carbon dioxide (scCO(2)) provides a different selectivity compared to that of conventional RP-UHPLC. The introduction of various hyphenation interfaces to MS broadens the applicability and sensitivity range of UHPSFC/MS using scCO(2) as the main component of the mobile phase. The addition of ionic additives (volatile salts, acids, and bases) to the modifier improves the separation of medium to polar lipids. Furthermore, UHPSFC/MS has also a great potential in the chiral separation of lipids, but the field has not yet been fully explored.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10406 - Analytical chemistry

Result continuities

  • Project

    <a href="/en/project/GA21-20238S" target="_blank" >GA21-20238S: Multidimensional chromatography - mass spectrometry quantitative workflows in detailed characterization of human plasma lipidome</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2022

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    TrAC - Trends in Analytical Chemistry

  • ISSN

    0165-9936

  • e-ISSN

    1879-3142

  • Volume of the periodical

    149

  • Issue of the periodical within the volume

    April

  • Country of publishing house

    NL - THE KINGDOM OF THE NETHERLANDS

  • Number of pages

    10

  • Pages from-to

    116546

  • UT code for WoS article

    000790927700001

  • EID of the result in the Scopus database

    2-s2.0-85124286290