Phenol/Chloroform-Free TiO2-Based miRNA Extraction from Cell Lysate
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F22%3A39919127" target="_blank" >RIV/00216275:25310/22:39919127 - isvavai.cz</a>
Alternative codes found
RIV/60162694:G44__/23:00558252
Result on the web
<a href="https://www.mdpi.com/1422-0067/23/16/8848" target="_blank" >https://www.mdpi.com/1422-0067/23/16/8848</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms23168848" target="_blank" >10.3390/ijms23168848</a>
Alternative languages
Result language
angličtina
Original language name
Phenol/Chloroform-Free TiO2-Based miRNA Extraction from Cell Lysate
Original language description
While microRNAs are considered as excellent biomarkers of various diseases, there are still several remaining challenges regarding their isolation. In this study, we aimed to design a novel RNA isolation method that would help to overcome those challenges. Therefore, we present a novel phenol/chloroform-free, low-cost method for miRNA extraction. Within this method, RNA is extracted from cell lysate with an isopropanol/water/NaCl system, followed by solid-phase extraction using TiO2 microspheres to effectively separate short RNAs from long RNA molecules. We also demonstrated the pH-dependent selectivity of TiO2 microspheres towards different sizes of RNA. We were able to regulate the size range of extracted RNAs with simple adjustments in binding conditions used during the solid-phase extraction.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/EF17_048%2F0007421" target="_blank" >EF17_048/0007421: Strengthening interdisciplinary cooperation in research of nanomaterials and their effects on living organisms (NANOBIO)</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1661-6596
e-ISSN
1422-0067
Volume of the periodical
23
Issue of the periodical within the volume
16
Country of publishing house
CH - SWITZERLAND
Number of pages
11
Pages from-to
8848
UT code for WoS article
000845731600001
EID of the result in the Scopus database
2-s2.0-85137739076