A new voltammetric approach for the determination of biomimetic catalyst kinetic constants based on substrate consumption
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216275%3A25310%2F23%3A39918941" target="_blank" >RIV/00216275:25310/23:39918941 - isvavai.cz</a>
Result on the web
<a href="https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elan.202200269" target="_blank" >https://analyticalsciencejournals.onlinelibrary.wiley.com/doi/epdf/10.1002/elan.202200269</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elan.202200269" target="_blank" >10.1002/elan.202200269</a>
Alternative languages
Result language
angličtina
Original language name
A new voltammetric approach for the determination of biomimetic catalyst kinetic constants based on substrate consumption
Original language description
In the present study, a new voltammetric method is introduced for the determination of kinetic parameters of artificial metalloenzymes (biomimetic complexes) mimicking the catecholase activity towards 3,5-di-tert-butylcatechol (3,5-DTBC) or 2,5-di-tert-butylhydroquinone, and representing a promising alternative to common spectrophotometric method. To demonstrate the effectiveness of the electrochemical approach, two binuclear copper(II) complexes bearing the same pentadentate ligand and different ionic ligands were selected. Apparent kinetic parameters, such as maximum velocity (V-max), Michaelis-Menten constant (Km), and turnover number (kcat), obtained by the described method employing differential-pulse voltammetry were in good agreement with the data evaluated from UV-Vis spectrophotometric measurements.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
<a href="/en/project/GA19-03160S" target="_blank" >GA19-03160S: Electrochemical study on new artificial enzymes and their role in sensing of neurotransmitters</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electroanalysis
ISSN
1040-0397
e-ISSN
1521-4109
Volume of the periodical
35
Issue of the periodical within the volume
3
Country of publishing house
DE - GERMANY
Number of pages
6
Pages from-to
"e202200269"
UT code for WoS article
000839576300001
EID of the result in the Scopus database
2-s2.0-85135912311