Advances in Digital Holographic Microscopy: Coherence-Controlled Microscope

Result code in IS VaVaI

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Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Advances in Digital Holographic Microscopy: Coherence-Controlled Microscope

Original language description

Digital holographic microscope (DHM) allows for direct accessing to the quantitative phase contrast. In this way it becomes a completely non-invasive tool for a contrast living cells observation and the dry-mass density distribution of a cell measurement. Current DHMs use the off-axis holographic setup based on conventional interferometers, which properly work with the coherent illumination. However, this type of illumination is inconvenient for microscopy because of the coherence noise, unwanted interference and a worse lateral resolution. An uncompromising solution to this problem is a DHM based on the Leith achromatic interferometer. While in this setup an off-axis hologram is formed with an arbitrary degree of the illumination coherence, the resolution and the image quality typical for an incoherent-light widefield microscopy can be achieved. Moreover, coherence gating can be introduced which makes the DHM image similar to that of confocal microscope. Hence the character of the DHM image is controlled by the coherence between two extremes: fully coherent-light holography and confocal-microscope-like imaging. The above described possibilities were proved experimentally by the coherence-controlled holographic microscope, which is a DHM based on the achromaticinterferometer. Living cancer cells were observed and their motility was evaluated in the quantitative phase contrast. The presence of the coherence gate was demonstrated by imaging of model samples through a scattering layer.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

BH - Optics, masers and lasers

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of SPIE

ISBN

9780819486646

ISSN

0277-786X

e-ISSN

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Number of pages

328

Pages from-to

"807402-1"-"807402-7"

Publisher name

SPIE-INT SOC OPTICAL ENGINEERING, 1000 20TH ST, PO BOX 10, BELLINGHAM, WA 98227-0010 USA

Place of publication

BELLINGHAM, USA

Event location

Praha

Event date

Apr 18, 2011

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000293696400001