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Studying of quantum dot luminescence quenching effect caused by covalent conjugation with protein

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F17%3APU124924" target="_blank" >RIV/00216305:26220/17:PU124924 - isvavai.cz</a>

  • Result on the web

    <a href="https://link.springer.com/content/pdf/10.1007%2Fs00706-017-2038-1.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007%2Fs00706-017-2038-1.pdf</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s00706-017-2038-1" target="_blank" >10.1007/s00706-017-2038-1</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Studying of quantum dot luminescence quenching effect caused by covalent conjugation with protein

  • Original language description

    The characterization of quantum dots (QDs) and their conjugates with bovine serum albumin was done using on-a-chip electrophoresis. Under optimal conditions, bioconjugated QDs were successfully separated from free QDs within approximate to10 min. Influence of coupling agent on a quenching of QD fluorescence intensity caused by conjugation to a protein was studied. When using two-step procedure combining 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide with N-hydroxysulfosuccinimide, the highest quenching effect was notable. We showed that QDs and capillary electrophoresis on a chip can be applied as a sensitive technique for the detection of biological molecules. The characteristics of this method are simplicity, small sample and reagent requirements, and high efficiency of separation.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Monatshefte für Chemie

  • ISSN

    0026-9247

  • e-ISSN

    1434-4475

  • Volume of the periodical

    148

  • Issue of the periodical within the volume

    11

  • Country of publishing house

    AT - AUSTRIA

  • Number of pages

    9

  • Pages from-to

    1901-1909

  • UT code for WoS article

    000413626000002

  • EID of the result in the Scopus database

    2-s2.0-85029548548