Studying of quantum dot luminescence quenching effect caused by covalent conjugation with protein
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F17%3APU124924" target="_blank" >RIV/00216305:26220/17:PU124924 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/content/pdf/10.1007%2Fs00706-017-2038-1.pdf" target="_blank" >https://link.springer.com/content/pdf/10.1007%2Fs00706-017-2038-1.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00706-017-2038-1" target="_blank" >10.1007/s00706-017-2038-1</a>
Alternative languages
Result language
angličtina
Original language name
Studying of quantum dot luminescence quenching effect caused by covalent conjugation with protein
Original language description
The characterization of quantum dots (QDs) and their conjugates with bovine serum albumin was done using on-a-chip electrophoresis. Under optimal conditions, bioconjugated QDs were successfully separated from free QDs within approximate to10 min. Influence of coupling agent on a quenching of QD fluorescence intensity caused by conjugation to a protein was studied. When using two-step procedure combining 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide with N-hydroxysulfosuccinimide, the highest quenching effect was notable. We showed that QDs and capillary electrophoresis on a chip can be applied as a sensitive technique for the detection of biological molecules. The characteristics of this method are simplicity, small sample and reagent requirements, and high efficiency of separation.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Monatshefte für Chemie
ISSN
0026-9247
e-ISSN
1434-4475
Volume of the periodical
148
Issue of the periodical within the volume
11
Country of publishing house
AT - AUSTRIA
Number of pages
9
Pages from-to
1901-1909
UT code for WoS article
000413626000002
EID of the result in the Scopus database
2-s2.0-85029548548