In Silico Prediction of Genes Coding Efflux Pumps in Clostridium Beijerinckii NRRL B-598

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F18%3APU128218" target="_blank" >RIV/00216305:26220/18:PU128218 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

In Silico Prediction of Genes Coding Efflux Pumps in Clostridium Beijerinckii NRRL B-598

Original language description

Predicting gene function can be very challenging. However, various in silico tools are available for this purpose. Here, we present a bioinformatics study focused on identifying potential genes that code efflux pumps in the strain Clostridium beijerinckii NRRL B-598. This rod-shaped, oxygen-tolerant, spore-forming bacterium is studied especially for its ability to produce acetone-butanol fermentation, with butanol being the main product. Current knowledge suggests that small uncharged molecules, including butanol, are transported out of the clostridial cell not only through a passive process of diffusion, but also due to the activity of efflux pumps. However, an ineffective excretion system together with low butanol tolerance currently present the most significant obstacles for industrial exploitation of butanol production in bacteria. Bioinformatics analysis of the strain C. beijerinckii NRRL B-598 can help us to understand these mechanisms in more detail not only for the studied strain, but also for Gram positive species in general. In this study, we used several online bioinformatics tools for the prediction of genes coding efflux pumps based on sequence information. Our results were verified by comparison with transcriptomic data using RNA-Seq technology. In silico identification of genes coding putative efflux pumps in C. beijerinckii NRRL B-598 will be further investigated in our future work, which will involve performing in vivo and in vitro lab experiments.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

20402 - Chemical process engineering

Project

<a href="/en/project/GA17-00551S" target="_blank" >GA17-00551S: Relationship between butanol efflux and butanol tolerance of Clostridia</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of the 6th International Conference on Chemical Technology (ICCT)

ISBN

978-80-86238-77-7

ISSN

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e-ISSN

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Number of pages

5

Pages from-to

86-90

Publisher name

Neuveden

Place of publication

Neuveden

Event location

Mikulov

Event date

Apr 16, 2018

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000461293800017