Diversity of Schlegelella thermodepolymerans Revealed with Nanopore Sequencing
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F22%3APU146521" target="_blank" >RIV/00216305:26220/22:PU146521 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Diversity of Schlegelella thermodepolymerans Revealed with Nanopore Sequencing
Original language description
Schlegelella thermodepolymerans is a thermophilic bacterium known for more than two decades thanks to its ability to decompose polyhydroxyalkanoates (PHA), environment-friendly, biodegradable and renewable alternatives to petrochemical polymers. Recently, we proved that the type strain S. thermodepolymerans DSM 15344T is also a potent PHA producer, which suggests its utilization for industrial production of bioplastics. Moreover, the strain prefers xylose over other sugars, including glucose. This is caused by unique xyl operon containing genes for xylose transport together with genes for its utilization as we found out from the complete genome assembly we had published previously. Here, we present genome comparisons of the type strain with additional strains S. thermodepolymerans DSM 15264, LMG 21645, and CCUG 50061 using nanopore sequencing. While genomes of all strains possessed gene machinery required for PHA metabolism, only strains DSM 15264 and LMG 21645 showed very high genome similarities, 97.9% and 96.1%, to the type strain, calculated as digital DNA-DNA hybridization (dDDH) values. On the contrary, the genome of the strain CCUG 50061 differed from the others not only in overall genome length but its dDDH value to the type strain was only 23.1%. Since the value is below the 70 % threshold for species delineation, the strain S. thermodepolymerans CCUG 50061 is probably misidentified. Nevertheless, resequencing with short reads is needed to polish inaccurate nanopore-based draft assemblies.
Czech name
—
Czech description
—
Classification
Type
D - Article in proceedings
CEP classification
—
OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/GA22-10845S" target="_blank" >GA22-10845S: Unraveling the role of polyhydroxyalkanoates in Schlegelella thermodepolymerans – promising environmental bacterium for next generation biotechnology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Proceedings of the 9th International Conference on Chemical Technology
ISBN
978-80-88307-11-2
ISSN
—
e-ISSN
—
Number of pages
6
Pages from-to
373-378
Publisher name
Czech Society of Industrial Chemistry
Place of publication
Prague
Event location
Mikulov
Event date
Apr 25, 2022
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
—