Bioinformatics study of the third generation sequencing platforms applied on a thermophile

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F24%3APU151251" target="_blank" >RIV/00216305:26220/24:PU151251 - isvavai.cz</a>

Result on the web

<a href="https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2024_sbornik_1.pdf" target="_blank" >https://www.eeict.cz/eeict_download/archiv/sborniky/EEICT_2024_sbornik_1.pdf</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Bioinformatics study of the third generation sequencing platforms applied on a thermophile

Original language description

This study compares the efficiency of Pacific Biosciences technology (PacBio) and Oxford Nanopore Technology (ONT) in sequencing, assembling, and annotating the Aneurinibacillus sp AFn2 bacterium. We aim to evaluate the performance based on contiguity, depth, and functional annotation of the resulting genome. Using ONT we generated 152,047 long reads assembling into 2 contigs with total base count of 0.4 billion which provided us efficient assembly while PacBio produced 139,701 reads, assembling into 21 contigs with a total base count of 1.4 billion. Functional annotation revealed differences in the number of coding sequences, with PacBio detecting more comprehensive gene sets than ONT. The comparative analysis done in this research shows the strengths and limitations of both the platforms, with ONT providing higher assembly contiguity and PacBio offering greater detail in genetic content. We aim to offer insights of both the sequencing technologies, guiding researchers in selecting the appropriate technology

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10201 - Computer sciences, information science, bioinformathics (hardware development to be 2.2, social aspect to be 5.8)

Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings I of the 30 th Conference STUDENT EEICT 2024

ISBN

978-80-214-6231-1

ISSN

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e-ISSN

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Number of pages

5

Pages from-to

202-205

Publisher name

Brno University of Technology, Faculty of Eletronic Engineering and Communication

Place of publication

Brno

Event location

Brno

Event date

Apr 23, 2024

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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