Machine learning based optimization for novel bio-ink development

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F26%3A0177751" target="_blank" >RIV/00216305:26220/26:0177751 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Machine learning based optimization for novel bio-ink development

Original language description

The quality of bio-ink, the backbone of almost every 3D-bioprinted construct, is one of the most critical aspects of successful 3D bioprinting. Various materials have been successfully used as bio-inks in 3D printing with promising biomedical applications. However, the formulation of printable bio-inks for extrusion-based 3D bioprinting remains a significant challenge in additive manufacturing. Bio-inks must demonstrate good mechanical properties, high biocompatibility, and proper printability to succeed in the chosen application. Moreover, identifying suitable printing conditions for new materials requires time-extensive and resource-demanding experimentation. Most recently, to accelerate bio-ink development, there has been significant attention towards the use of artificial intelligence techniques in this process. Combining machine learning with high-throughput theoretical predictions and high-throughput experiments has altered the traditional trial and error paradigm into a data-driven paradigm. In this study, a novel bio-ink is developed composed of chitosan, gelatin, and agarose using a machine learning method to accelerate the fabrication process. The bio-ink is examined for its printability, rheological properties, hydrophilicity, degradability, and biological response. Rheological analysis displayed that the viscosity of the optimized bio-ink was in a suitable range that facilitated reproducible and reliable printing. Various 3D constructs with different layer orientations were fabricated to test their printability and shape fidelity. The ink was then exposed to mesenchymal bone marrow stem cells (MSCs) to evaluate cell adhesion, growth, and morphology on the surface. The morphological study of the cells showed that they were alive and well grown on the bio-ink. Further characterization using MTT assay demonstrated that cells were still viable on the printed construct after subjecting to physiological conditions for three days. These results suggested that the bio-ink may be a potential biomaterial suitable for use in 3D complex tissue constructs fabrication.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of IUPESM World Congress on Medical Physics and Biomedical Engineering XXVII

ISBN

978-3-032-20290-1

ISSN

1680-0737

e-ISSN

1433-9277

Number of pages

13

Pages from-to

1-13

Publisher name

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Place of publication

Singapore

Event location

Singapore

Event date

Jun 12, 2022

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

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