Sub-nL thin-film differential scanning calorimetry chip for rapid thermal analysis of liquid samples

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F26%3A0186839" target="_blank" >RIV/00216305:26220/26:0186839 - isvavai.cz</a>

Result on the web

<a href="https://www.sciencedirect.com/science/article/abs/pii/S0956566323002610?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/abs/pii/S0956566323002610?via%3Dihub</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1039/d2lc01094a" target="_blank" >10.1039/d2lc01094a</a>

Result language

angličtina

Original language name

Sub-nL thin-film differential scanning calorimetry chip for rapid thermal analysis of liquid samples

Original language description

Differential scanning calorimetry (DSC) is a popular thermal analysis technique. The miniaturization of DSC on chip as thin-film DSC (tfDSC) has been pioneered for the analysis of ultrathin polymer films at temperature scan rates and sensitivities far superior to those attainable with DSC instruments. The adoption of tfDSC chips for the analysis of liquid samples is, however, confronted with various issues including sample evaporation due to the lack of sealed enclosures. Although the subsequent integration of enclosures has been demonstrated in various designs, rarely did those designs exceed the scan rates of DSC instruments mainly because of their bulky features and requirement for exterior heating. Here, we present a tfDSC chip featuring sub-nL thin-film enclosures integrated with resistance temperature detectors (RTDs) and heaters. The chip attains an unprecedented sensitivity of 11 V W-1 and a rapid time constant of 600 ms owing to its low-addenda design and residual heat conduction (similar to 6 mu W K-1). We present results on the phase transition of common liquid crystals which we leverage to calibrate the RTDs and characterize the thermal lag with scan rates up to 900 degrees C min(-1). We then present results on the heat denaturation of lysozyme at various pH values, concentrations, and scan rates. The chip can provide excess heat capacity peaks and enthalpy change steps without much alteration induced by the thermal lag at elevated scan rates up to 100 degrees C min(-1), which is an order of magnitude faster than those of many chip counterparts.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10600 - Biological sciences

Project

<a href="/en/project/VI04000057" target="_blank" >VI04000057: An ultrafast portable system to detect SARS-CoV-2</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Lab on a chip

ISSN

1473-0197

e-ISSN

1473-0189

Volume of the periodical

23

Issue of the periodical within the volume

7

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

1926-1934

UT code for WoS article

000945329800001

EID of the result in the Scopus database

2-s2.0-85149894631