Low-power microfluidic RT-LAMP system with real-time fluorescence detection for portable nucleic acid testing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26220%2F26%3A0200011" target="_blank" >RIV/00216305:26220/26:0200011 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007/s00604-025-07680-2" target="_blank" >https://link.springer.com/article/10.1007/s00604-025-07680-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00604-025-07680-2" target="_blank" >10.1007/s00604-025-07680-2</a>

Result language

angličtina

Original language name

Low-power microfluidic RT-LAMP system with real-time fluorescence detection for portable nucleic acid testing

Original language description

A microfluidic reverse transcription loop-mediated isothermal amplification (RT-LAMP) platform is presented featuring integrated real-time fluorescence detection and optimized thermal control. Magnetic bead-based RNA extraction was paired with a thermally insulated reaction chip operated under vacuum to suppress heat loss. Additional energy-saving strategies, including reduced wire cross-section and low-emissivity surface treatment, reduced total power consumption from (2.00 +/- 0.08) W to (0.29 +/- 0.01) W, corresponding to an 85.5 % reduction. Amplification was completed within 20 min, and positive fluorescence signals were detected in less than 11 min. Although LAMP reagents remain more expensive than those used in PCR, the substantial energy savings and system simplification demonstrated here support practical battery-powered operation. The RT-LAMP chemistry used in this system matched a previously validated configuration, which achieved a limit of detection of 256 RNA copies and a signal slope of (- 1.70 +/- 0.20) dec.min(-)1. These parameters remained consistent under the current setup, as power consumption did not influence detection sensitivity provided the reaction temperature was maintained. The platform enables low-power, real-time nucleic acid detection suited for decentralized diagnostics, forensic screening, and environmental monitoring.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/TN02000017" target="_blank" >TN02000017: National Centre for Biotechnology in Veterinary Medicine</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2025

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

MICROCHIMICA ACTA

ISSN

0026-3672

e-ISSN

1436-5073

Volume of the periodical

1

Issue of the periodical within the volume

193

Country of publishing house

AT - AUSTRIA

Number of pages

12

Pages from-to

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UT code for WoS article

001644808100001

EID of the result in the Scopus database

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