Fully Automated Ancestral Sequence Reconstruction using FireProtASR
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26230%2F21%3APU138920" target="_blank" >RIV/00216305:26230/21:PU138920 - isvavai.cz</a>
Result on the web
<a href="https://currentprotocols.onlinelibrary.wiley.com/doi/full/10.1002/cpz1.30" target="_blank" >https://currentprotocols.onlinelibrary.wiley.com/doi/full/10.1002/cpz1.30</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cpz1.30" target="_blank" >10.1002/cpz1.30</a>
Alternative languages
Result language
angličtina
Original language name
Fully Automated Ancestral Sequence Reconstruction using FireProtASR
Original language description
Protein evolution and protein engineering techniques are of great interest in basic science and industrial applications such as pharmacology, medicine, or biotechnology. Ancestral sequence reconstruction (ASR) is a powerful technique for probing evolutionary relationships and engineering robust proteins with good thermostability and broad substrate specificity. The following protocol describes the setting up and execution of an automated FireProtASR workflow using a dedicated web site. The service allows for inference of ancestral proteins automatically, from a single protein sequence. Once a protein sequence is submitted, the server will build a dataset of homology sequences, perform a multiple sequence alignment (MSA), build a phylogenetic tree, and reconstruct ancestral nodes. The protocol is also highly flexible and allows for multiple forms of input, advanced settings, and the ability to start jobs from: (i) a single sequence, (ii) a set of homologous sequences, (iii) an MSA, and (iv) a phylogenetic tree. This approach automates all necessary steps and offers a way for novices with limited exposure to ASR techniques to improve the properties of a protein of interest. The technique can even be used to introduce catalytic promiscuity into an enzyme. A web server for accessing the fully automated workflow is freely accessible at https://loschmidt.chemi.muni.cz/fireprotasr/
Czech name
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Czech description
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Classification
Type
J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database
CEP classification
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OECD FORD branch
10201 - Computer sciences, information science, bioinformathics (hardware development to be 2.2, social aspect to be 5.8)
Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Current Protocols in Bioinformatics
ISSN
1934-3396
e-ISSN
1934-340X
Volume of the periodical
1
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
5
Pages from-to
1-5
UT code for WoS article
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EID of the result in the Scopus database
2-s2.0-85102178516