The application of PCR methods and gas chromatography for detection of specific non-pathogenic bacteria of the genus Clostridium

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F09%3APU87649" target="_blank" >RIV/00216305:26310/09:PU87649 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
The application of PCR methods and gas chromatography for detection of specific non-pathogenic bacteria of the genus Clostridium
Original language description
The aim of this study was to select and investigate non-pathogenic bacterial strains of the genus Clostridium collected in the Dairy Research Institute. The strains were isolated from cheeses with late blowing disorders. First, a set of 44 strains was identified into the genus Clostridium using genus-specific PCR (Rekha et al., 2006). PCRs specific to the species Clostridium butyricum (Nakanishi et al., 2005) and Clostridium tyrobutyricum (Herman et al., 1995) were used for species identification. It was found that 7 and 12 strains belonged to the species C. butyricum or C. tyrobutyricum, respectively. Some strains were not identified into species yet. Strains encoding the H2-evolving hydrogenase (hydA) gene were also identified using PCR ( ). Formation of fatty acids (acetic, propionic, and butyric) was studied by gas chromatography in culture supernatants.
Czech name
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Czech description
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Project
<a href="/en/project/2B08070" target="_blank" >2B08070: Development of new modern biotechnological process for more effective utilization of waste water from dairy industry as material source for hydrogen production.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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