Analysis of Vitamin D in feed by UPLC-MS/MS
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F13%3APU105791" target="_blank" >RIV/00216305:26310/13:PU105791 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Analysis of Vitamin D in feed by UPLC-MS/MS
Original language description
Various forms of vitamin D, namely cholecalciferol (vitamin D3), ergocalciferol (vitamin D2) and 25-hydroxycholecalciferol, are included in the European Union Register of Feed Additives. Maximum limits for these compounds have been established and vary depending on the feed [1, 2]. The simultaneous use of different forms of vitamin D is prohibited or strictly directed. Central Institute for Supervising and Testing in Agriculture inspects the animal feedstuffs, premixtures and mineral feeds for various parameters including the content of cholecalciferol. Method for determination of vitamin D based on BS EN 12821:2009 [3] has been validated. Sample preparation is based on liquid-liquid extraction after alkaline saponification and determination is carriedout by ultra-performance liquid chromatography coupled to tandem mass spectrometry using positive electrospray ionization (UPLC-MS/MS). Deuterated cholecalciferol is used as internal standard for the quantification of cholecalciferol in
Czech name
—
Czech description
—
Classification
Type
O - Miscellaneous
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
—
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů