Application of random mutagenesis to enhance the production of polyhydroxyalkanoates by Cupriavidus necator H16 on waste frying oil

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F13%3APU106754" target="_blank" >RIV/00216305:26310/13:PU106754 - isvavai.cz</a>
Alternative codes found
RIV/60193697:_____/13:#0000830
Result on the web
<a href="http://dx.doi.org/10.1007/s11274-013-1410-5" target="_blank" >http://dx.doi.org/10.1007/s11274-013-1410-5</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11274-013-1410-5" target="_blank" >10.1007/s11274-013-1410-5</a>

Result language
angličtina
Original language name
Application of random mutagenesis to enhance the production of polyhydroxyalkanoates by Cupriavidus necator H16 on waste frying oil
Original language description
Using random chemical mutagenesis we obtained the mutant of Cupriavidus necator H16 which was capable of improved (about 35 %) production of poly(3-hydroxybuytrate) (PHB) compared to the wild-type strain. The mutant exhibited significantly enhanced specific activities of enzymes involved in oxidative stress response such as malic enzyme, NADP-dependent isocitrate dehydrogenase, glucose-6-phosphate dehydrogenase and glutamate dehydrogenase. Probably, due to the activation of these enzymes, we also observed an increase of NADPH/NADP? ratio. It is likely that as a side effect of the increase of NADPH/NADP ratio the activity of PHBbiosynthetic pathwaywas enhanced,which supported the accumulation of PHB. Furthermore, the mutant was also able to incorporatepropionate into copolymer poly(3-hydroxybuytyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)] more efficiently than the wild-type strain (Y3HV/prec = 0.17 and 0.29 for the wild-type strain and themutant, respectively)).We assume that it may be
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Project
<a href="/en/project/ED0012%2F01%2F01" target="_blank" >ED0012/01/01: Centres for materials research at FCH BUT</a><br>
Continuities
O - Projekt operacniho programu

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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