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The use of gas chromatography for determination of residual tert-butanol in dried systems of hyaluronana and fluorescence probes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F14%3APU110375" target="_blank" >RIV/00216305:26310/14:PU110375 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    The use of gas chromatography for determination of residual tert-butanol in dried systems of hyaluronana and fluorescence probes

  • Original language description

    Hyaluronan is a native biopolymer with hydrophilic character, which contains on its chain also hydrophobic areas. Due to the prevalent hydrophilicity these parts are protected by organized water molecules. For accessing the hydrophobic parts for interactions with hydrophobic fluorescence probes the method of drying was used. During the preparation of samples for drying tert-butanol was added. The problem arise if residual part of tert-butanol remains in the sample due to its hydrophobic organic character, which can significantly affect the result of fluorescence measurement. The amount of residual tert-butanol was determined by head-space extraction with gas chromatography detection.

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

    CF - Physical chemistry and theoretical chemistry

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů