Portable medium pressure capillary liquid chromatography based on a modular microfluidic system using off-the-shelf components

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F15%3APU118829" target="_blank" >RIV/00216305:26310/15:PU118829 - isvavai.cz</a>
Result on the web
<a href="http://pittcon.org/pittcon-2015/" target="_blank" >http://pittcon.org/pittcon-2015/</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Portable medium pressure capillary liquid chromatography based on a modular microfluidic system using off-the-shelf components
Original language description
Here we investigated a design philosophy of a portable medium-pressure LC system based on using off-the-shelf easily accessible components. The basis of the system is a modular microfluidic platform with the components assembled in a flexible manner on abreadboard (Fig.1). The components are syringe pumps and switch valves, joined with capillaries and microfluidic fittings, complemented with other components, including an injection valve and on-capillary detectors, all controlled by a PC. The pumping system is formed by miniaturised syringe pumps (5, 20 and 100?L options). Two pairs of pumps were used for each mobile phase to enable gradient elution capability, connected with two microfluidic switching valves, and then connected together through a Y-connector, thus providing a low hold-up volume for gradient formation. A 4 or 20 nL nano-LC sampling valve was used for the injection system, connecting to a capillary LC column (100 ?m i.d., 50- 250 mm length, C18 RP monolith). Photometri
Czech name
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Czech description
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Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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