Phase Transition of Lipids Membranes Determination: Comparison of Different Methods
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F16%3APU119098" target="_blank" >RIV/00216305:26310/16:PU119098 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Phase Transition of Lipids Membranes Determination: Comparison of Different Methods
Original language description
In this work we compare results of phase transition temperature measurements by different methods: microcalorimetry and fluorescence spectroscopy. For this purpose, we prepared the series of lipid mixtures consisting of cationic and zwitterionic lipids with different lipid ratios. Measurements of phase transition temperature by steady-state fluorescence spectroscopy can be utilized prodan and its derivative laurdan. These fluorescence probes are sensitive to the polarity of local environment due to partial charge separation between the 2-dimethylamino and the 6-carbonyl residues forming dipole moment, which causes solvent reorientation upon excitation. This solvent reorientation consumes energy and decreases the probe's excited state energy resulting in continuous red shift of the probe's emission spectrum. Thus, this shift in laurdan and prodan emission spectra indicates the increased concentration of water within the bilayer and its increased mobility.The laurdan in compared to prodan contains lauri
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů