Effect of Hyaluronan on Diffusion Properties of Artificial Cell Membrane Studied by Fluorescence Correlation Spectroscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F16%3APU120655" target="_blank" >RIV/00216305:26310/16:PU120655 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Effect of Hyaluronan on Diffusion Properties of Artificial Cell Membrane Studied by Fluorescence Correlation Spectroscopy
Original language description
Supported lipid bilayers were used as simplified models of cell membranes to study the effect of hyaluronan solution on their diffusion properties. The bilayers were prepared from lecithin liposomes in phosphate buffer using the vesicle fusion method. Hydrophilized cover glass slides were chosen as the supporting substrate. Two fluorescence probes – Nile Red and DiO were utilized to quantify the diffusion properties of the bilayer with and without presence of hyaluronan. For this purpose Z-scan Fluorescence Correlation Spectroscopy, which is a method developed specially for planar samples, was used. It was found out, that presence of this biopolymer causes slowing of the fluorescence probe diffusion within the bilayer.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LO1211" target="_blank" >LO1211: Materials Research Centre at FCH BUT- Sustainability and Development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
XVI. Workshop of Physical Chemists and Electrochemists
ISBN
978-80-210-8267-0
ISSN
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e-ISSN
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Number of pages
4
Pages from-to
135-138
Publisher name
Masaryk University, Brno
Place of publication
Brno
Event location
Brno
Event date
Jun 6, 2016
Type of event by nationality
WRD - Celosvětová akce
UT code for WoS article
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