Adaptation of Cupriavidus necator H16 to levulinic acid for enhancement of 3-hydroxyvalerate content in copolymer P(3HB-co-3HV)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F18%3APU129873" target="_blank" >RIV/00216305:26310/18:PU129873 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Adaptation of Cupriavidus necator H16 to levulinic acid for enhancement of 3-hydroxyvalerate content in copolymer P(3HB-co-3HV)

Original language description

Within this work we used methods of evolutionary engineering to gain strains adapted to levulinic acid able to growth at the presence of levulinic acid and to convert it to 3HV and incorporate into copolymer P(3HB-co-3HV) more than wild-type strain. On the basis of specific enzyme activities of selected enzymes through metabolism of evolved strains we tried to suggest possible metabolic adaptation strategies and we also used methods of statistical analysis such as PCA and AHC for searching of common and different strategies between evolved strains.

Czech name

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Czech description

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Type

O - Miscellaneous

CEP classification

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OECD FORD branch

20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials

Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů