Effects of hydration of sodium 3-hydroxybutyrate on phase transitions connected to cryoprotectivity of microorganisms
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F18%3APU129876" target="_blank" >RIV/00216305:26310/18:PU129876 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Effects of hydration of sodium 3-hydroxybutyrate on phase transitions connected to cryoprotectivity of microorganisms
Original language description
In this work, we are focused on cryoprotective mechanisms utilized by bacterial strain Cupriavidus necator H16 which produces and accumulates polyhydroxyalkanoates (PHA) in form of intracellular granules because of storage of carbon and energy and innate ability to protect itself against stress conditions. But intracellular granules do not obtain only PHA, due to metabolism of the PHA where PHA is simultaneously synthesized and degraded, the granules also contain its monomer 3-hydroxybutyrate (3HB).In our experimental part, we used differential scanning calorimetry (DSC) to observe the glass transitions and separated them into one independent and two dependent regions of phase diagram on water content. Also using the DSC data, we established the amount of nonfreezing water, which was calculated from linear regression (Figure 1) to be approx. 1,22g/g of monomer Na3HB. For determination of sorption isotherms of Na3HB with various content of water, we measured water activity by sorption calorimetry, dyna
Czech name
—
Czech description
—
Classification
Type
O - Miscellaneous
CEP classification
—
OECD FORD branch
20903 - Bioproducts (products that are manufactured using biological material as feedstock) biomaterials, bioplastics, biofuels, bioderived bulk and fine chemicals, bio-derived novel materials
Result continuities
Project
—
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů