The influence of quadruplex structure in proximity to p53 target sequences on the transactivation potential of p53alpha isoforms
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F19%3APU133880" target="_blank" >RIV/00216305:26310/19:PU133880 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/20:00524258
Result on the web
<a href="https://www.mdpi.com/1422-0067/21/1/127" target="_blank" >https://www.mdpi.com/1422-0067/21/1/127</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms21010127" target="_blank" >10.3390/ijms21010127</a>
Alternative languages
Result language
angličtina
Original language name
The influence of quadruplex structure in proximity to p53 target sequences on the transactivation potential of p53alpha isoforms
Original language description
p53 is one of the most studied tumor suppressor proteins that plays an important role in basic biological processes including cell cycle, DNA damage response, apoptosis and senescence. The human TP52 gene contains alternative promoters that produce N-terminally truncated proteins and can produce several isoforms due to alternative splicing. p53 function is realized by binding to a specific DNA response element (RE), resulting in the transactivation of target genes. Here, we evaluated the influence of quadruplex DNA structure in the transactivation potential of full-lenght and N-terminal truncated p43alpha isoforms in a panel of S. cerevisiae luciferase reporter strains. Our results show that a G-quadruplex prone sequence is not sufficient for transcription activation by p53alpha isoforms, but the presence of this feature in proximity to a p53 RE leads to a significant reduction of transcriptional activity and changes the dynamics between co-expressed p53alpha isoforms.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Molecular Sciences
ISSN
1422-0067
e-ISSN
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Volume of the periodical
21
Issue of the periodical within the volume
1
Country of publishing house
CH - SWITZERLAND
Number of pages
12
Pages from-to
1-15
UT code for WoS article
000515378000127
EID of the result in the Scopus database
2-s2.0-85077247199