Study of Metabolic Adaptation of Red Yeasts to Waste Animal Fat Substrate

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F19%3APU134553" target="_blank" >RIV/00216305:26310/19:PU134553 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2076-2607/7/11/578" target="_blank" >https://www.mdpi.com/2076-2607/7/11/578</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/microorganisms7110578" target="_blank" >10.3390/microorganisms7110578</a>

Result language

angličtina

Original language name

Study of Metabolic Adaptation of Red Yeasts to Waste Animal Fat Substrate

Original language description

Carotenogenic yeasts are non-conventional oleaginous microorganisms capable to utilize various waste substrates. In this work 4 red yeast strains (Rhodotorula, Cystofilobasidium and Sporobolomyces sp.) were cultivated in media containing crude, emulsified and enzymatically hydrolysed animal waste fat, compared with glucose and glycerol as single C-sources. Cell morphology (cryo-SEM, TEM), production of biomass, lipase, biosurfactants, lipids (GC/FID) carotenoids, ubiquinone, ergosterol (HPLC/PDA) in yeast cells was studied depending on medium composition, C-source and C/N ratio. All studied strains are able to utilize solid and processed fat. Biomass production at C/N=13 was higher on emulsified/hydrolysed fat than on glucose/glycerol. Production of lipids and lipidic metabolites was enhanced for several times on fat; the highest yields of carotenoids (24.8 mg/l) and lipids (54.5%/CDW) were found in S.pararoseus. Simultaneous induction of lipase and biosurfactants was observed on crude fat substrate. Increased C/N ratio (13-100) led to higher biomass production in fat media. Production of total lipids increased in all strains to C/N 50. Oppositely, production of carotenoids, ubiquinone and ergosterol dramatically decreased with increased C/N in all strains. Compounds accumulated in stressed red yeasts are having great application potential and can result from valorization of animal waste fat in the biorefinery concept.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10609 - Biochemical research methods

Project

—

Continuities

R - Projekt Ramcoveho programu EK

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Microorganisms

ISSN

2076-2607

e-ISSN

—

Volume of the periodical

7

Issue of the periodical within the volume

11

Country of publishing house

CH - SWITZERLAND

Number of pages

28

Pages from-to

1-28

UT code for WoS article

000502273600101

EID of the result in the Scopus database

2-s2.0-85075323754