Attenuation of p53-alpha isoform transactivation by inverted repeat sequences in p53 target sites

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F19%3APU136159" target="_blank" >RIV/00216305:26310/19:PU136159 - isvavai.cz</a>
Result on the web
<a href="https://2019.febscongress.org/abstract_preview.aspx?idAbstractEnc=4424170094096098094092424170" target="_blank" >https://2019.febscongress.org/abstract_preview.aspx?idAbstractEnc=4424170094096098094092424170</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Attenuation of p53-alpha isoform transactivation by inverted repeat sequences in p53 target sites
Original language description
p53 is one of the most studied tumour suppressor proteins, playing important roles in regulating basic biological processes including cell cycle, apoptosis, senescenceand metabolism. The human Tp53 gene contains alternative promoters and thanks to alternative splicing can produce several isoforms. p53 protein function is realizedby binding to specific DNA response elements resulting in the transactivation of target genes. Here we present results of p53alpha isoform obtained using a yeastisogenic system for in vivo transactivation studies in chromosomal context to specifically evaluate the influence of secondary DNA structures on transactivation. We useda panel of S. cerevisiaehaploid strains that are isogenic except for different p53 DNA binding sites positioned upstream of a luciferase reporter gene and chosen basedon different propensities to form DNA structures. The targeting of the chosen p53 binding site was achieved by the Delitto Perfettooligonucleotide targeting techniqueby the replacement of
Czech name
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Czech description
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Project
<a href="/en/project/LO1211" target="_blank" >LO1211: Materials Research Centre at FCH BUT- Sustainability and Development</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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