Newly designed primers targeting limonene synthase

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F21%3APU139903" target="_blank" >RIV/00216305:26310/21:PU139903 - isvavai.cz</a>
Result on the web
<a href="https://www.tandfonline.com/doi/full/10.1080/13102818.2020.1871545?fbclid=IwAR0gLYRgommM-eySZC1qddUErA0VWyAlsj5S0gYm3QG8A0HLbrOJyJyu624" target="_blank" >https://www.tandfonline.com/doi/full/10.1080/13102818.2020.1871545?fbclid=IwAR0gLYRgommM-eySZC1qddUErA0VWyAlsj5S0gYm3QG8A0HLbrOJyJyu624</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Newly designed primers targeting limonene synthase
Original language description
Cosmetic products with ingredients of plant origin promise beneficial effects on the customer health. Those may contain allergens that belong to the group of 24 fragrance allergens that can negatively affect the health of sensitive individuals. One of these allergens is d-limonene present for example in citrus fruits. The presence of plant DNA in the cosmetic product can be detected using techniques of molecular biotechnology, even if it is present in very small quantity. New primers Lim2 F/R, targeting limonene synthase, were designed to confirm the presence of the citrus DNA in a leave-on cosmetic product. The DNA of Citrus limon was isolated from the leave-on cosmetic product and the specific sequence was amplified using real-time PCR. The presence of amplicons of specific size was confirmed using agarose electrophoresis.
Czech name
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Czech description
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Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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