Polarity-Based Sequential Extraction as a Simple Tool to Reveal the Structural Complexity of Humic Acids

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F21%3APU140474" target="_blank" >RIV/00216305:26310/21:PU140474 - isvavai.cz</a>

Result on the web

<a href="https://www.mdpi.com/2073-4395/11/3/587" target="_blank" >https://www.mdpi.com/2073-4395/11/3/587</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3390/agronomy11030587" target="_blank" >10.3390/agronomy11030587</a>

Result language

angličtina

Original language name

Polarity-Based Sequential Extraction as a Simple Tool to Reveal the Structural Complexity of Humic Acids

Original language description

A sequential chemical extraction with a defined series of eluotropic organic solvents with an increasing polarity (trichloromethane < ethyl acetate < acetone < acetonitrile < n-propanol < methanol) was performed on peat-bog humic acid. Six organic fractions were obtained and subjected to a physicochemical characterization utilizing methods of structural and compositional analysis. Advanced spectroscopic techniques such as Attenuated Total Reflectance (ATR-FTIR), total luminescence, and liquid-state 13C NMR spectrometry were combined with elemental analysis of the organic fractions. In total, the procedure extracted about 57% (wt.) of the initial material; the individual fractions amounted from 1.1% to 19.7%. As expected, the apolar solvents preferentially released lipid-like components, while polar solvents provided organic fractions rich in oxygen-containing polar groups with structural parameters closer to the original humic material. The fraction extracted with acetonitrile shows distinct structural features with its lower aromaticity and high content of protein-like structural motifs. The last two—alcohol extracted—fractions show the higher content of carbohydrate residues and their specific (V-type) fluorescence suggests the presence of plant pigment residues. The extraction procedure is suggested for further studies as a simple but effective way to decrease the structural complexity of a humic material enabling its detail and more conclusive compositional characterization.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10403 - Physical chemistry

Project

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Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Agronomy

ISSN

2073-4395

e-ISSN

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Volume of the periodical

11

Issue of the periodical within the volume

3

Country of publishing house

CH - SWITZERLAND

Number of pages

19

Pages from-to

1-19

UT code for WoS article

000633184900001

EID of the result in the Scopus database

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