Increasing PHA production in cyanobacteria by mixotrophic cultivation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F21%3APU142442" target="_blank" >RIV/00216305:26310/21:PU142442 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Increasing PHA production in cyanobacteria by mixotrophic cultivation
Original language description
Cyanobacteria are phototrophic gram-negative bacteria capable of oxygenic photosynthesis. They are able to synthetize polyhydroxyalkanoates (PHA). They do not require costly organic substrate and, oppositely, cyanobacteria can fix CO2. PHA serve as carbon and energy source in form of intracellular granules. PHA help cyanobacteria to survive stress conditions. In this work we demonstrated that stress conditions lead to the highest yields of PHA. Two cultures from genera Synechocystis were used. Multiple stressors were applied on cultures, stressors include PHA precursors. Cultivation with the same PHA precursors in same concentration led to 1,6-higher amount of PHA when multicultivator was used. The highest amount of PHA reached the 25 % of PHA in dry biomass. Strains are capable of copolymer synthesis in presence of its precursor. Synechocystis sp. PCC 6803 cultivated with γ-butyrolactone accumulated copolymer of 3-hydroxybutyrate (44 %) and 4-hydroxybutyrate (56 %). The total amount of PHA as well as
Czech name
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Czech description
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Project
<a href="/en/project/GF19-29651L" target="_blank" >GF19-29651L: The ecological role of poly-hydroxybutyrate in cyanobacteria</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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