Use of Flavin-Related Cellular Autofluorescence to Monitor Processes in Microbial Biotechnology
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F22%3APU145818" target="_blank" >RIV/00216305:26310/22:PU145818 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/2076-2607/10/6/1179/htm" target="_blank" >https://www.mdpi.com/2076-2607/10/6/1179/htm</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/microorganisms10061179" target="_blank" >10.3390/microorganisms10061179</a>
Alternative languages
Result language
angličtina
Original language name
Use of Flavin-Related Cellular Autofluorescence to Monitor Processes in Microbial Biotechnology
Original language description
Cellular autofluorescence is usually considered to be a negative phenomenon because it can affect the sensitivity of fluorescence microscopic or flow cytometric assays by interfering with the signal of various fluorescent probes. Nevertheless, in our work, we adopted a different approach, and green autofluorescence induced by flavins was used as a tool to monitor fermentation employing the bacterium Cupriavidus necator. The autofluorescence was used to distinguish microbial cells from abiotic particles in flow cytometry assays, and it was also used for the determination of viability or metabolic characteristics of the microbial cells. The analyses using two complementary techniques, namely fluorescence microscopy and flow cytometry, are simple and do not require labor sample preparation. Flavins and their autofluorescence can also be used in a combination with other fluorophores when the need for multi-parametrical analyses arises, but it is wise to use dyes that do not emit a green light in order to not interfere with flavins’ emission band (500–550 nm).
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/GA19-20697S" target="_blank" >GA19-20697S: Directed evolution of polyhydroxyalkanoates producing bacteria</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microorganisms
ISSN
2076-2607
e-ISSN
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Volume of the periodical
10
Issue of the periodical within the volume
6
Country of publishing house
CH - SWITZERLAND
Number of pages
21
Pages from-to
1-21
UT code for WoS article
000930526300001
EID of the result in the Scopus database
2-s2.0-85131573880