Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F24%3APU154851" target="_blank" >RIV/00216305:26310/24:PU154851 - isvavai.cz</a>

Result on the web

<a href="https://link.springer.com/article/10.1007/s12223-024-01212-w#citeas" target="_blank" >https://link.springer.com/article/10.1007/s12223-024-01212-w#citeas</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s12223-024-01212-w" target="_blank" >10.1007/s12223-024-01212-w</a>

Result language

angličtina

Original language name

Evaluating stress resilience of cyanobacteria through flow cytometry and fluorescent viability assessment

Original language description

Cyanobacteria are prokaryotic organisms characterised by their complex structures and a wide range of pigments. With their ability to fix CO2, cyanobacteria are interesting for white biotechnology as cell factories to produce various high-value metabolites such as polyhydroxyalkanoates, pigments, or proteins. White biotechnology is the industrial production and processing of chemicals, materials, and energy using microorganisms. It is known that exposing cyanobacteria to low levels of stressors can induce the production of secondary metabolites. Understanding of this phenomenon, known as hormesis, can involve the strategic application of controlled stressors to enhance the production of specific metabolites. Consequently, precise measurement of cyanobacterial viability becomes crucial for process control. However, there is no established reliable and quick viability assay protocol for cyanobacteria since the task is challenging due to strong interferences of autofluorescence signals of intercellular pigments and fluorescent viability probes when flow cytometry is used. We performed the screening of selected fluorescent viability probes used frequently in bacteria viability assays. The results of our investigation demonstrated the efficacy and reliability of three widely utilised types of viability probes for the assessment of the viability of Synechocystis strains. The developed technique can be possibly utilised for the evaluation of the importance of polyhydroxyalkanoates for cyanobacterial cultures with respect to selected stressor-repeated freezing and thawing. The results indicated that the presence of polyhydroxyalkanoate granules in cyanobacterial cells could hypothetically contribute to the survival of repeated freezing and thawing.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

20800 - Environmental biotechnology

Project

<a href="/en/project/GF19-29651L" target="_blank" >GF19-29651L: The ecological role of poly-hydroxybutyrate in cyanobacteria</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2024

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

FOLIA MICROBIOLOGICA

ISSN

0015-5632

e-ISSN

1874-9356

Volume of the periodical

70

Issue of the periodical within the volume

jaro

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

19

Pages from-to

„“-„“

UT code for WoS article

001349486800001

EID of the result in the Scopus database

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