An Initial Genome Editing Toolset for <i>Caldimonas thermodepolymerans</i>, the First Model of Thermophilic Polyhydroxyalkanoates Producer
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26310%2F26%3A0197285" target="_blank" >RIV/00216305:26310/26:0197285 - isvavai.cz</a>
Result on the web
<a href="https://enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/1751-7915.70103" target="_blank" >https://enviromicro-journals.onlinelibrary.wiley.com/doi/10.1111/1751-7915.70103</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1111/1751-7915.70103" target="_blank" >10.1111/1751-7915.70103</a>
Alternative languages
Result language
angličtina
Original language name
An Initial Genome Editing Toolset for <i>Caldimonas thermodepolymerans</i>, the First Model of Thermophilic Polyhydroxyalkanoates Producer
Original language description
The limited number of well-characterised model bacteria cannot address all the challenges in a circular bioeconomy. Therefore, there is a growing demand for new production strains with enhanced resistance to extreme conditions, versatile metabolic capabilities and the ability to utilise cost-effective renewable resources while efficiently generating attractive biobased products. Particular thermophilic microorganisms fulfil these requirements. Non-virulent Gram-negative Caldimonas thermodepolymerans DSM15344 is one such attractive thermophile that efficiently converts a spectrum of plant biomass sugars into high quantities of polyhydroxyalkanoates (PHA)-a fully biodegradable substitutes for synthetic plastics. However, to enhance its biotechnological potential, the bacterium needs to be 'domesticated'. In this study, we established effective homologous recombination and transposon-based genome editing systems for C. thermodepolymerans. By optimising the electroporation protocol and refining counterselection methods, we achieved significant improvements in genetic manipulation and constructed the AI01 chassis strain with improved transformation efficiency and a Delta phaC mutant that will be used to study the importance of PHA synthesis in Caldimonas. The advances described herein highlight the need for tailored approaches when working with thermophilic bacteria and provide a springboard for further genetic and metabolic engineering of C. thermodepolymerans, which can be considered the first model of thermophilic PHA producer.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
20801 - Environmental biotechnology
Result continuities
Project
<a href="/en/project/GA22-10845S" target="_blank" >GA22-10845S: Unraveling the role of polyhydroxyalkanoates in Schlegelella thermodepolymerans – promising environmental bacterium for next generation biotechnology</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Microbial Biotechnology
ISSN
1751-7915
e-ISSN
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Volume of the periodical
18
Issue of the periodical within the volume
2
Country of publishing house
US - UNITED STATES
Number of pages
16
Pages from-to
1-16
UT code for WoS article
001426536600001
EID of the result in the Scopus database
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