Isolation, detection and quantification of Hepatitis B Virus using magnetic nanobeads and chip electrophoresis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F11%3APU107637" target="_blank" >RIV/00216305:26620/11:PU107637 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Isolation, detection and quantification of Hepatitis B Virus using magnetic nanobeads and chip electrophoresis
Original language description
In this study, magnetic nanobeads coated with streptavidine were prepared to isolate ssDNA specific to Hepatitis B virus (HBV). DNA probes labeled by biotine molecules were designed to be complementary to specific HBV genes. Artificial synthetic oligonucleotides (25?35 nt), identical to HBV gene sequences, were used to mimic a real sample of human blood serum. The amount of nanoparticles utilized for isolation target molecules was within the range from 1 to 20 uL. It was found that already 1 uL of MNPswas sufficient for effective isolation of the molecule of the interest.
Czech name
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Czech description
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Classification
Type
O - Miscellaneous
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů