Isolation of Xis Gen Fragment of lambda Phage from Agarose Gel Using Magnetic Particles for Subsequent Enzymatic DNA Sequencing

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F12%3APU100297" target="_blank" >RIV/00216305:26620/12:PU100297 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/13:00213487

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Isolation of Xis Gen Fragment of lambda Phage from Agarose Gel Using Magnetic Particles for Subsequent Enzymatic DNA Sequencing

Original language description

Gel electrophoresis is one of the most important methods used in biochemistry and molecular biology. The recovery of analytes from the gel required for subsequent analysis including amplification by polymerase chain reaction (PCR) or DNA sequencing is anissue due to the gel contamination. Among the other methods used for sample recovery, the application of nanomaterials is also being investigated. In this study, the applicability of magnetic particles (1 lm) for isolation of DNA fragment from agarose gel with subsequent DNA sequencing was investigated. Electrochemical analysis and DNA sequencing was used to investigate the recovery yield. The influence of dilution of the gel prior to the purification was investigated and the linear dependence with regression coefficient R2 = 0.9972 was obtained using square wave voltammetry. Moreover, bioinformatic analysis was used for comparison of obtained sequences, and simple and easy identification of nonsystematic errors caused by both fluoresc

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

CHROMATOGRAPHIA

ISSN

0009-5893

e-ISSN

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Volume of the periodical

2012

Issue of the periodical within the volume

10

Country of publishing house

DE - GERMANY

Number of pages

6

Pages from-to

1-6

UT code for WoS article

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EID of the result in the Scopus database

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