Sequences of pandemic-causing viruses isolated and detected by paramagnetic particles coupled with microfluidic system and electrochemical detector

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F13%3APU104844" target="_blank" >RIV/00216305:26620/13:PU104844 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/13:00204831

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Sequences of pandemic-causing viruses isolated and detected by paramagnetic particles coupled with microfluidic system and electrochemical detector

Original language description

Detection of the specific sequences of pandemic-causing viral nucleic acids is area attracting numerous scientists worldwide. In this study, we focused on the isolation and detection of oligonucleotide specific sequences of life-threatening viruses (HIV(GAGCAGTGGGAATA), Influenza (TAATAACCATTGGA) and Ebola (ACCTCACTAGAAAA)) by flow injection analysis with electrochemical detection coupled with paramagnetic particles based isolation. All of used viral genomic sequences contained A6 adenine repetition onthe 5 end. This modification enables us to bind them on paramagnetic particle anchoring T25 (MPs-(dT)25). The isolated sequences were subsequently hydrolysed by optimized method using microwaves to obtain single adenines. The obtained samples were detected by microfluidic tool based on stopped flow injection analysis with glassy carbon electrode in the presence of Cu(II)/or CuS nanoparticles to enhance sensitivity and to lower detection limit for adenine. Basic detection parameters as a

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

ISSN

1452-3981

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

12

Country of publishing house

RS - THE REPUBLIC OF SERBIA

Number of pages

15

Pages from-to

12628-12642

UT code for WoS article

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EID of the result in the Scopus database

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