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Single Amino Acid Change in Metallothionein Metal-Binding Cluster Influences Interaction with Cisplatin

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F13%3APU105675" target="_blank" >RIV/00216305:26620/13:PU105675 - isvavai.cz</a>

  • Alternative codes found

    RIV/62156489:43210/13:00213567 RIV/00216208:11310/13:10135334 RIV/00216208:11130/13:10135334 RIV/00064203:_____/13:10135334

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Single Amino Acid Change in Metallothionein Metal-Binding Cluster Influences Interaction with Cisplatin

  • Original language description

    The issue of tumour cell resistance to anticancer drugs is a major problem in the treatment of this grave disease and it is still not satisfactorily explained. Its base lies in the interaction of a cytostatic with biomolecules synthesized by tumour cells. One of the generally accepted mechanisms of resistance to some metal based cytostatics is the overexpression of metallothionein in tumour cells. In this study, electrochemical profile of interaction between 23 sulphur-rich fragments of the metal-binding protein metallothionein and cisplatin was studied. To evaluate the results, interaction constants were suggested. Here, we found that the maximum increased interaction (more than 100 %) occurred, when conservative aminoacids were substituted for more than one position outside the cysteine cluster. On the contrary, amino acid substitution within the cysteine cluster led to a reduction in interaction constants (up to 10-25% of average). This result clearly indicates that aminoacids outside cysteine binding motif are of high importance for interactions of metallothionein with cisplatin.Based on the results it can be assumed that the substitution of individual aminoacids in the peptide chain of protein markedly influences the interaction with cisplatin, which could be used for designing new types of cytostatics.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CG - Electrochemistry

  • OECD FORD branch

Result continuities

  • Project

    Result was created during the realization of more than one project. More information in the Projects tab.

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2013

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE

  • ISSN

    1452-3981

  • e-ISSN

  • Volume of the periodical

    8

  • Issue of the periodical within the volume

    2

  • Country of publishing house

    RS - THE REPUBLIC OF SERBIA

  • Number of pages

    10

  • Pages from-to

    2625-2634

  • UT code for WoS article

  • EID of the result in the Scopus database