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Dynamic phase differences method for the assessment of cellular dynamic processes

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F14%3APU113982" target="_blank" >RIV/00216305:26620/14:PU113982 - isvavai.cz</a>

  • Result on the web

    <a href="http://www.ceitec.eu/ac2014-book-of-abstract/f32414" target="_blank" >http://www.ceitec.eu/ac2014-book-of-abstract/f32414</a>

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Dynamic phase differences method for the assessment of cellular dynamic processes

  • Original language description

    Imaging of cellular mass transfers and therefore the traits of live cell behaviour is nowadays a rewarding task in life sciences. As a tool for revealing those dynamic processes, we present a method of dynamic phase differences (DPD). DPD fully exploitsthe quantitative phase images obtained by the Coherence-Controlled Holographic Microscope (CCHM) developed in Brno University of Technology. DPD method is employed for the objective evaluation of the growth, migration and motility of cells, which would not be obvious only from the quantitative phase images. The results can be directly visualised by the 2D colour image, which could lead to identification of typical patterns characterizing the behaviour of cells. The method also provides the opportunity to calculate the transferred mass of the cell over a distance and therefore enables to quantitatively measure the movements of the cell (in picograms).

  • Czech name

  • Czech description

Classification

  • Type

    O - Miscellaneous

  • CEP classification

    BH - Optics, masers and lasers

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach

Others

  • Publication year

    2014

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů