On-chip three-dimensional cell culture in phaseguides improves hepatocyte functions in vitro

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F15%3APU114563" target="_blank" >RIV/00216305:26620/15:PU114563 - isvavai.cz</a>

Result on the web

<a href="http://scitation.aip.org/content/aip/journal/bmf/9/3/10.1063/1.4922863" target="_blank" >http://scitation.aip.org/content/aip/journal/bmf/9/3/10.1063/1.4922863</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1063/1.4922863" target="_blank" >10.1063/1.4922863</a>

Result language

angličtina

Original language name

On-chip three-dimensional cell culture in phaseguides improves hepatocyte functions in vitro

Original language description

The in vitro study of liver functions and liver cell specific responses to external stimuli deals with the problem to preserve the in vivo functions of primary hepatocytes. In this study, we used the biochip OrganoPlateTM (MIMETAS) that combines different advantages for the cultivation of hepatocytes in vitro: (1) the perfusion flow is achieved without a pump allowing easy handling and placement in the incubator; (2) the phaseguides allow plating of matrix-embedded cells in lanes adjacent to the perfusion flow without physical barrier; and (3) the matrix-embedding ensures indirect contact of the cells to the flow. In order to evaluate the applicability of this biochip for the study of hepatocyte’s functions, MatrigelTM-embedded HepG2 cells were cultured over three weeks in this biochip and compared to a static Matrigel culture (3D) and a monolayer culture (2D). Chip-cultured cells grew in spheroid-like structures and were characterized by the formation of bile canaliculi and a high viability over 14 days. Hepatocyte-specific physiology was achieved as determined by an increase in albumin production. Improved detoxification metabolism was demonstrated by strongly increased cytochrome P450 activity and urea production. Additionally, chip-cultured cells displayed increased sensitivity to cetaminophen. Altogether, the OrganoPlate seems to be a very useful alternative for the cultivation of hepatocytes, as their behavior was strongly improved over 2D and static 3D cultures and the results were largely comparable and partly superior to the previous reports on biochip-cultured hepatocytes. As for the low technical needs, this platform has the appearance ofbeing highly applicable for further studies of hepatocytes’ responses to external stimuli. stimuli.VC 2015 AIP Publishing LLC.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

20601 - Medical engineering

Project

<a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomicrofluidics

ISSN

1932-1058

e-ISSN

—

Volume of the periodical

9

Issue of the periodical within the volume

034113

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

„034113-1“-„034113-13“

UT code for WoS article

000357090100016

EID of the result in the Scopus database

2-s2.0-84933055140