Industrial Tomography System for Answering Biological Issues: Development of the Mouse Embryo Face.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F16%3APU118925" target="_blank" >RIV/00216305:26620/16:PU118925 - isvavai.cz</a>

Result on the web

<a href="http://www.ndt.net/search/docs.php3?id=18758" target="_blank" >http://www.ndt.net/search/docs.php3?id=18758</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Industrial Tomography System for Answering Biological Issues: Development of the Mouse Embryo Face.

Original language description

Understanding of some biological processes like a face development requires among others the high-resolution 3D imaging of increasingly complex cartilage in verterate embryos. During the face development, cartilages are produced in a variety of shapes and sizes making a convenient model system. X-ray computed microtomography (microCT) imaging is limited by the low inherent contrast of non-mineralized tissues. Although X-ray contrast enhancement agents are used routinely in clinical radiography, only a few techniques have appeared for imaging soft tissues in preserved animal specimens. Here we present a feasibility study of three different tomographic systems for high resolution and high contrast imaging of embryonic tissues. The industrial system equipped with flat-panel is compared with microCT system using synchrotron radiation (SYRMEP beamline of Elettra, Italy) and laboratory-based micro/nano CT system (Rigaku, nano3DX) based on Mo target and CCD camera. The mouse embryos were stained by phosphotungstic acid which produced overall contrast and differential tissue contrast. Furthermore, we demonstrate the utilisation of the standard industrial tools (wall thickness analysis and 3D printing) which can help to understand the differences or similarities among of different mouse embryonic development stages.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

JR - Other machinery industry

OECD FORD branch

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Project

<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

The e-Journal of Nondestructive Testing

ISSN

1435-4934

e-ISSN

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Volume of the periodical

Neuveden

Issue of the periodical within the volume

2016

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

1-9

UT code for WoS article

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EID of the result in the Scopus database

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