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Superficial cells are self-renewing chondrocyte progenitors, which form the articular cartilage in juvenile mice

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F17%3APU121490" target="_blank" >RIV/00216305:26620/17:PU121490 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1096/fj.201600918R" target="_blank" >http://dx.doi.org/10.1096/fj.201600918R</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1096/fj.201600918R" target="_blank" >10.1096/fj.201600918R</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Superficial cells are self-renewing chondrocyte progenitors, which form the articular cartilage in juvenile mice

  • Original language description

    Articular cartilage has little regenerative capacity. Recently, genetic lineage tracing experiments have revealed chondrocyte progenitors at the articular surface. We further characterized these progenitors by using in vivo genetic approaches. Histone H2B–green fluorescent protein retention revealed that superficial cells divide more slowly than underlying articular chondrocytes. Clonal genetic tracing combined with immunohistochemistry revealed that superficial cells renew their number by symmetric division, express mesenchymal stem cell markers, and generate chondrocytes via both asymmetric and symmetric differentiation. Quantitative analysis of cellular kinetics, in combination with phosphotungstic acid–enhanced micro–computed tomography,showed that superficial cells generate chondrocytes and contribute to the growth and reshaping of articular cartilage. Furthermore, we found that cartilage renewal occurs as the progeny of superficial cells fully replace fetal chondrocytes during early postnatal life. Thus, superficial cells are self-renewing progenitors that are capable of maintaining their own population and fulfilling criteria of unipotent adult stem cells. Furthermore, the progeny of these cells reconstitute adult articular cartilage de novo, entirely substituting fetal chondrocytes.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    <a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    The FASEB Journal

  • ISSN

    0892-6638

  • e-ISSN

    1530-6860

  • Volume of the periodical

    31

  • Issue of the periodical within the volume

    3

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    18

  • Pages from-to

    1067-1084

  • UT code for WoS article

    000395671200021

  • EID of the result in the Scopus database

    2-s2.0-85014580341