Use of the industrial X-ray computed microtomography to address scientific questions in developmental biology

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F19%3APU131922" target="_blank" >RIV/00216305:26620/19:PU131922 - isvavai.cz</a>

Result on the web

<a href="https://www.ndt.net/search/docs.php3?showForm=off&id=23689" target="_blank" >https://www.ndt.net/search/docs.php3?showForm=off&id=23689</a>

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Use of the industrial X-ray computed microtomography to address scientific questions in developmental biology

Original language description

X-ray computed microtomography (microCT) that allows to extract quantitative 3D information about various objects is already a well-established and widely used industrial technique for non-destructive testing, materials characterization and dimensional metrology. On the other hand, modern developmental biology requires both qualitative and quantitative three-dimensional information about the studied objects, which is not provided by conventional 2D imaging methods. In addition, the complexity of biological structures often requires a more comprehensive methodology to compare shapes and sizes. Our approach is to connect these two worlds, i.e. to apply procedures and analysis routinely used or developed for industrial computed tomography to address scientific questions in developmental biology. This approach, which is based on the long-term experience of the CEITEC BUT Brno micro/nanoCT research team on both, industrial and scientific application of X-ray microCT, brings new possibilities in imaging, data processing and evaluation of 3D biological models. Here we present 3D structures that were analysed by different approaches on the selected examples from developmental biology. In addition, quantitative analysis usually used in industry were applied to segmented 3D models e.g. wall thickness analysis of facial cartilage, shape comparisons of nasal capsules, automatic quantification of the cells in the salamander limb etc. A complete procedure consisting of staining, microCT measurement and subsequent data processing is discussed.

Czech name

—

Czech description

—

Type

J_ost - Miscellaneous article in a specialist periodical

CEP classification

—

OECD FORD branch

20602 - Medical laboratory technology (including laboratory samples analysis; diagnostic technologies) (Biomaterials to be 2.9 [physical characteristics of living material as related to medical implants, devices, sensors])

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

The e-Journal of Nondestructive Testing

ISSN

1435-4934

e-ISSN

—

Volume of the periodical

24

Issue of the periodical within the volume

3

Country of publishing house

DE - GERMANY

Number of pages

9

Pages from-to

1-9

UT code for WoS article

—

EID of the result in the Scopus database

—