Tuning LC-MS/MS analysis for identification of peptide extracts from cryosections of porcine lung tissue affected by Actinobacillus pleuropneumoniae

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F19%3APU134873" target="_blank" >RIV/00216305:26620/19:PU134873 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/19:43917124
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Tuning LC-MS/MS analysis for identification of peptide extracts from cryosections of porcine lung tissue affected by Actinobacillus pleuropneumoniae
Original language description
The porcine pleuropneumonia belongs to common pig bacterial infection caused by Actinobacillus pleuropneumoniae. This disease is affecting the economy of pig breeding. Therefore, it is important to better understand its effects and to find targeted therapy. To elucidate this infection different approaches can be used. One of them is to monitor the response of organism and detect changed expressions of proteins connected to immune reaction, especially cytokines subgroup called interleukins. Usually, these proteins are detected by enzyme-linked immunosorbent assay (ELISA), but other methods like label-free liquid chromatography connected with tandem mass spectrometry (LCMS/MS) can be used. This study was aimed at adapting/developing a high-performance liquid chromatography-electrospray ionisation quadrupole time-of-flight mass spectrometry (HPLC-ESIQqTOF MS) method for analysing these infection markers after on-tissue digestion and extraction from the surface of porcine lung tissue sections.
Czech name
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Czech description
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2019 Proceedings of 26th International PhD Students Conference
ISBN
978-80-7509-688-3
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
557-562
Publisher name
Neuveden
Place of publication
Neuveden
Event location
Brno
Event date
Nov 6, 2019
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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