Effect of sarcosine dehydrogenase knockdown on sarcosine metabolism-related genes expression

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F19%3APU134930" target="_blank" >RIV/00216305:26620/19:PU134930 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/19:43917151
Result on the web
<a href="https://mendelnet.cz/artkey/mnt-201901-0125_Effect-of-sarcosine-dehydrogenase-knockdown-on-sarcosine-metabolism-related-genes-expression.php?back=/magno/mnt/2019/mn1.php?secid=4" target="_blank" >https://mendelnet.cz/artkey/mnt-201901-0125_Effect-of-sarcosine-dehydrogenase-knockdown-on-sarcosine-metabolism-related-genes-expression.php?back=/magno/mnt/2019/mn1.php?secid=4</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Effect of sarcosine dehydrogenase knockdown on sarcosine metabolism-related genes expression
Original language description
Sarcosine is extensively discussed as potential prostate cancer oncometabolite. Sarcosine dehydrogenase (SARDH) is one of the key enzymes involved in sarcosine metabolism that catalyses oxidative demethylation of sarcosine to glycine in mitochondrion. This study investigates the effects of small interfering RNA (siRNA) mediated SARDH knockdown on gene expression of sarcosine metabolism related enzymes: glycine N-methyltransferase (GNMT), peroxisomal sarcosine oxidase (PIPOX) and dimethylglycine dehydrogenase (DMGDH). The effect of SARDH knockdown was studied in three human prostate cell lines (PNT1A, DU-145, PC3) and gene expression was evaluated by quantitative real-time PCR (qPCR). Although the lowest SARDH knockdown was achieved in cancer DU-145 cell line, the highest changes in expression of other sarcosine metabolism genes were detected. In particular, the significant increase in DMGDH and PIPOX expression was observed. Our findings revealed potential stimulation effect of SARDH knockdown on DMGDH and PIPOX expression in prostate cancer cell line DU-145.
Czech name
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Czech description
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Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2019 Proceedings of 26th International PhD Students Conference
ISBN
978-80-7509-688-3
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
648-653
Publisher name
Mendel University in Brno
Place of publication
neuveden
Event location
Brno
Event date
Nov 6, 2019
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
000576735500117

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