Determining spatial distribution of interleukin-1 beta as an infection marker in pulmonary porcine tissues

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F19%3APU140135" target="_blank" >RIV/00216305:26620/19:PU140135 - isvavai.cz</a>
Result on the web
<a href="https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf" target="_blank" >https://mnet.mendelu.cz/mendelnet2019/mnet_2019_full.pdf</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Determining spatial distribution of interleukin-1 beta as an infection marker in pulmonary porcine tissues
Original language description
Interleukin-1 beta (IL-1 beta) belongs among proinflammatory cytokines that are produced by activated macrophages as an immune response of the organism. Therefore, IL-1 beta could serve as a possible biomarker of a bacterial infection caused by Actinobacillus pleuropneumonia. In this study, we applied matrix assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) together with immunohistochemical analyses (IHC) using primary antibody anti-IL-1 beta for IL-1 beta detection in cryo-sectioned pulmonary tissues. Using MALDI MSI, we detected and spatially visualized the tryptic fragment of IL-1 beta. This IL-1 beta tryptic fragment was more frequently detected in infected pulmonary tissues compared to healthy pulmonary tissue except one necrotic lung tissue where was not revealed the statistically significant difference. IHC confirmed the occurrence of IL-1 beta in necrotic pulmonary tissues. This knowledge could be useful for further clinical studying as a targeted therapy.
Czech name
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Czech description
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Project
<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
MendelNet 2019: Proceedings of International PhD Students Conference
ISBN
978-80-7509-688-3
ISSN
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e-ISSN
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Number of pages
6
Pages from-to
459-464
Publisher name
Neuveden
Place of publication
Neuveden
Event location
Brno
Event date
Nov 6, 2019
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
000576735500082

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