Heat Transfer Time Determination Based on DNA Melting Curve Analysis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F20%3APU132821" target="_blank" >RIV/00216305:26620/20:PU132821 - isvavai.cz</a>
Result on the web
<a href="https://link.springer.com/article/10.1007/s10404-019-2308-9" target="_blank" >https://link.springer.com/article/10.1007/s10404-019-2308-9</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10404-019-2308-9" target="_blank" >10.1007/s10404-019-2308-9</a>

Result language
angličtina
Original language name
Heat Transfer Time Determination Based on DNA Melting Curve Analysis
Original language description
The determination of the physical properties of fluids – such as the thermal characteristics, which include heat transfer time (Δt) – is becoming more challenging as system sizes shrink to micro and nanometer scales. Hence, knowledge of these properties is crucial for the operation of devices requiring precise temperature (T) control, such as polymerase chain reactions, melting curve analysis (MCA), and differential scanning fluorimetry. In this paper, we introduced a flow-through microfluidic system to analyze and compare thermal properties such as Δt among samples and the sidewall of a silicon chip using microscopic image analysis. We performed a spatial MCA with double-stranded deoxynucleic acid (dsDNA) and EvaGreen intercalator, using a flow-through microfluidic chip, and achieved a T gradient of ≈ 2.23 K·mm−1. We calculated the mean value of Δt as ≈ 33.9 ms from a melting temperature (TM) location shift along the microchannel for a variable flow rate. Our system had a T resolution of ≈ 1.2 mK·pixel-1 to distinguish different dsDNA molecules – based on the TM location within the chip – providing an option to use it as a high-throughput device for rapid DNA or protein analysis.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
21002 - Nano-processes (applications on nano-scale); (biomaterials to be 2.9)

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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