Metallothionein-3 promotes cisplatin chemoresistance remodelling in neuroblastoma

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F21%3APU140501" target="_blank" >RIV/00216305:26620/21:PU140501 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/21:43919375 RIV/00064203:_____/21:10425777 RIV/00216208:11130/21:10425777
Result on the web
<a href="https://www.nature.com/articles/s41598-021-84185-x" target="_blank" >https://www.nature.com/articles/s41598-021-84185-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-021-84185-x" target="_blank" >10.1038/s41598-021-84185-x</a>

Result language
angličtina
Original language name
Metallothionein-3 promotes cisplatin chemoresistance remodelling in neuroblastoma
Original language description
Metallothionein-3 has poorly characterized functions in neuroblastoma. Cisplatin-based chemotherapy is a major regimen to treat neuroblastoma, but its clinical efficacy is limited by chemoresistance. We investigated the impact of human metallothionein-3 (hMT3) up-regulation in neuroblastoma cells and the mechanisms underlying the cisplatin-resistance. We confirmed the cisplatin-metallothionein complex formation using mass spectrometry. Overexpression of hMT3 decreased the sensitivity of neuroblastoma UKF-NB-4 cells to cisplatin. We report, for the first time, cisplatin-sensitive human UKF-NB-4 cells remodelled into cisplatin-resistant cells via high and constitutive hMT3 expression in an in vivo model using chick chorioallantoic membrane assay. Comparative proteomic analysis demonstrated that several biological pathways related to apoptosis, transport, proteasome, and cellular stress were involved in cisplatin-resistance in hMT3 overexpressing UKF-NB-4 cells. Overall, our data confirmed that up-regulation of hMT3 positively correlated with increased cisplatin-chemoresistance in neuroblastoma, and a high level of hMT3 could be one of the causes of frequent tumour relapses.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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