Peptide-based electrochemical biosensors utilized for protein detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F21%3APU140681" target="_blank" >RIV/00216305:26620/21:PU140681 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/21:43919637

Result on the web

<a href="https://www.sciencedirect.com/science/article/pii/S095656632100124X" target="_blank" >https://www.sciencedirect.com/science/article/pii/S095656632100124X</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bios.2021.113087" target="_blank" >10.1016/j.bios.2021.113087</a>

Result language

angličtina

Original language name

Peptide-based electrochemical biosensors utilized for protein detection

Original language description

Proteins are generally detected as biomarkers for tracing or determining various disorders in organisms. Biomarker proteins can be tracked in samples with various origins and in different concentrations, revealing whether an organism is in a healthy or unhealthy state. In regard to detection, electrochemical biosensors are a potential fusion of electronics, chemistry, and biology, allowing for fast and early point-of-care detection from a biological sample with the advantages of high sensitivity, simple construction, and easy operation. Peptides present a promising approach as a biorecognition element when connected with electrochemical biosensors. The benefits of short peptides lie mainly in their good stability and selective affinity to a target analyte. Therefore, peptide-based electrochemical biosensors (PBEBs) represent an alternative approach for the detection of different protein biomarkers. This review provides a summary of the past decade of recently proposed PBEBs designed for protein detection, dividing them according to different protein types: (i) enzyme detection, including proteases and kinases; (ii) antibody detection; and (iii) other protein detection. According to these protein types, different sensing mechanisms are discussed, such as the peptide cleavage by a proteases, phosphorylation by kinases, presence of antibodies, and exploiting of affinities; furthermore, measurements are obtained by different electrochemical methods. A discussion and comparison of various constructions, modifications, immobilization strategies and different sensing techniques in terms of high sensitivity, selectivity, repeatability, and potential for practical application are presented.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

BIOSENSORS & BIOELECTRONICS

ISSN

0956-5663

e-ISSN

1873-4235

Volume of the periodical

180

Issue of the periodical within the volume

113087

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

19

Pages from-to

1-19

UT code for WoS article

000634110200001

EID of the result in the Scopus database

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