Paperfluidic devices with a selective molecularly imprinted polymer surface for instrumentation-free distance-based detection of protein biomarkers

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F21%3APU140946" target="_blank" >RIV/00216305:26620/21:PU140946 - isvavai.cz</a>

Alternative codes found

RIV/62156489:43210/21:43919859

Result on the web

<a href="https://doi.org/10.1016/j.snb.2021.129999" target="_blank" >https://doi.org/10.1016/j.snb.2021.129999</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.snb.2021.129999" target="_blank" >10.1016/j.snb.2021.129999</a>

Result language

angličtina

Original language name

Paperfluidic devices with a selective molecularly imprinted polymer surface for instrumentation-free distance-based detection of protein biomarkers

Original language description

Microfluidic paper-based analytical devices (mu PADs) offer the advantages of simplicity, extremely low costs, robustness, and miniaturisation, synergistically supporting portability and point-of-care (POC) analysis. When mu PADs are combined with distance-based detection in D mu PADs, they uniquely enable a quantitative analytical platform that is truly instrumentation-free (naked-eye readout), or at least, does not require any specialised scientific instrumentation (only mobile phone camera). However, a significant drawback of D mu PADs is their limited selectivity. In this work, we present for the first time molecularly imprinted polymer (MIP) as a selectivity-enhancing element in MIP-modified D mu PADs (MIP-D mu PADs). Herein, a layer of polydopamine MIP was coated onto the paper substrate of a D mu PAD, in a simple process using dopamine as the monomer deposited onto the paper matrix in the migration-detection zone of the D mu PAD, and polymerised in a rapid low cost procedure in the presence of oxygen under alkaline conditions. The polydopamine MIP-D mu PAD was then systematically investigated for the selective determination of chymotrypsinogen (chymo) as a model protein biomarker in urine, within the linear concentration range 2.4-29.2 mu M (R2 = 0,9903) with corresponding relative standard deviations ranging from 2% to 11 % and LOD =3.5 mu M and LOQ =11.8 mu M. The here presented analytical concept based on MIP-D mu PADs has a potential in POC diagnostics, because of the combination of low cost automated fabrication, the rapid quantitative near to instrumentation-free analysis, and selectivity through the use of MIPs as a synthetic, more stable, cheaper and easily prepared alternative to bio-macromolecules.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2021

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Sensors and Actuators B: Chemical

ISSN

0925-4005

e-ISSN

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Volume of the periodical

341

Issue of the periodical within the volume

129999

Country of publishing house

CH - SWITZERLAND

Number of pages

10

Pages from-to

1-10

UT code for WoS article

000652632200001

EID of the result in the Scopus database

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