Resolving complex cartilage structures in developmental biology via deep learning-based automatic segmentation of X-ray computed microtomography images
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F22%3APU144599" target="_blank" >RIV/00216305:26620/22:PU144599 - isvavai.cz</a>
Result on the web
<a href="https://www.nature.com/articles/s41598-022-12329-8" target="_blank" >https://www.nature.com/articles/s41598-022-12329-8</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-022-12329-8" target="_blank" >10.1038/s41598-022-12329-8</a>
Alternative languages
Result language
angličtina
Original language name
Resolving complex cartilage structures in developmental biology via deep learning-based automatic segmentation of X-ray computed microtomography images
Original language description
The complex shape of embryonic cartilage represents a true challenge for phenotyping and basic understanding of skeletal development. X-ray computed microtomography (mu CT) enables inspecting relevant tissues in all three dimensions; however, most 3D models are still created by manual segmentation, which is a time-consuming and tedious task. In this work, we utilised a convolutional neural network (CNN) to automatically segment the most complex cartilaginous system represented by the developing nasal capsule. The main challenges of this task stem from the large size of the image data (over a thousand pixels in each dimension) and a relatively small training database, including genetically modified mouse embryos, where the phenotype of the analysed structures differs from the norm. We propose a CNN-based segmentation model optimised for the large image size that we trained using a unique manually annotated database. The segmentation model was able to segment the cartilaginous nasal capsule with a median accuracy of 84.44% (Dice coefficient). The time necessary for segmentation of new samples shortened from approximately 8 h needed for manual segmentation to mere 130 s per sample. This will greatly accelerate the throughput of mu CT analysis of cartilaginous skeletal elements in animal models of developmental diseases.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10700 - Other natural sciences
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
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Volume of the periodical
12
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
13
Pages from-to
1-13
UT code for WoS article
000799975800023
EID of the result in the Scopus database
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